Step (1) | 10 mins at 22 °C; 10 mins at 60 °C; Or 5 mins at 95 °C | lysis of virus-containing patient sample using QuickExtract to release viral RNA; |
Step (2) | 1 hr at 60 °C | detection of viral RNA using STOPCovid reaction; |
Step (3) | 2 mins at 22 °C | visual read out of the detection result by eye using a commercially-available paper dipstick. |