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[Preprint]. 2020 May 8:2020.05.04.20091231. [Version 1] doi: 10.1101/2020.05.04.20091231
Initial concentration Final concentration Amount (uL)
Isothermal amplification buffer 10 X 1 X 5
dNTPs 10 mM 1.4 mM 7
MgSO4 100 mM 8 mM 4
WS Bst 2.0 8000 units/mL 320 units/mL 2
WS RTx 15,000 units/mL 300 units/mL 1
Aap Cas12b 2 mg/mL or 15.4 uM 500 nM 1.625
Aap Cas12b crRNA 360 ng/uL or 10 uM 500 nM 2.5
Lateral Flow Reporter 100 uM 125 nM 0.0625
Taurine 500 mM 50 mM 5
LAMP primer mix 10 X 1 X 5
ddH2O 11.8125
Total 45