Initial concentration | Final concentration | Amount (uL) | |
---|---|---|---|
Isothermal amplification buffer | 10 X | 1 X | 5 |
dNTPs | 10 mM | 1.4 mM | 7 |
MgSO4 | 100 mM | 8 mM | 4 |
WS Bst 2.0 | 8000 units/mL | 320 units/mL | 2 |
WS RTx | 15,000 units/mL | 300 units/mL | 1 |
Aap Cas12b | 2 mg/mL or 15.4 uM | 500 nM | 1.625 |
Aap Cas12b crRNA | 360 ng/uL or 10 uM | 500 nM | 2.5 |
Lateral Flow Reporter | 100 uM | 125 nM | 0.0625 |
Taurine | 500 mM | 50 mM | 5 |
LAMP primer mix | 10 X | 1 X | 5 |
ddH2O | 11.8125 | ||
Total | 45 |