Table 1.
Converged mutations identified in the clones of acid-adapted strains at pH 5.5
Flask number stands for the number of cell passages during the evolution. Numbers in bold represent the final flask number (and thus the endpoint) in the evolution process of the specific acid-adapted strain. Non-bold numbers are intermediate flasks during the evolution where samples were selected for whole genome sequencing.
|
Gene |
Mutation |
Protein change |
Flask number |
AA1 |
AA2 |
AA3 |
AA4 |
AA5 |
AA6 |
|---|---|---|---|---|---|---|---|---|---|
|
rho |
C→A |
R102S (CGC→AGC) |
87 |
X |
|||||
|
rho |
C→T |
R102C (CGC→TGC) |
111 |
|
X |
||||
|
rpoC |
C→A |
A397E (GCG→GAG) |
111 |
|
X |
||||
|
rpoC |
G→C |
G444A (GGT→GCT) |
88, 118 |
|
X |
||||
|
rpoC |
Δ7 bp |
coding (4106 −4112/4224 nt) |
113 |
|
X |
||||
|
rpoC |
Δ1 bp |
coding (4111/4224 nt) |
111 |
|
X |
||||
|
rpoC |
C→T |
S539F (TCT→TTT) |
111 |
|
X |
||||
|
nagA |
G→C |
S90* (TCA→TGA) |
84 |
|
X |
||||
|
nagA |
C→T |
R149H (CGT→CAT) |
83 |
|
X |