Antibodies |
|
|
anti-Total Oxphos (mouse) |
Abcam |
Cat# ab110413; RRID: AB_2629281 |
anti-COXIV (rabbit) |
Cell Signaling |
Cat# 4850S; RRID: AB_2085424 |
anti-rabbit secondary (donkey) |
Li-Cor |
RRID: AB_2715510 |
anti-mouse secondary (donkey) |
Li-Cor |
RRID: AB_2716622 |
Chemicals, Peptides, and Recombinant Proteins |
D-[U-13C]-glucose |
Cambridge Isotope Laboratories |
Cat# CLM-1396 |
D-[U-13C]-malate |
Cambridge Isotope Laboratories |
Cat# CLM-8065 |
D-[4-2H]-glucose |
Omicron Biochemicals |
Cat# GLC-035 |
[U-13C]-succinic acid |
Sigma |
Cat# 491985 |
Experimental Models: Organisms/Strains |
Mouse: C57BL/6J |
Jackson Laboratory |
RRID: IMSR_JAX:000664 |
Mouse: AIPL1−/−
|
Ramamurthy et. al 2004 (Hurley Lab) |
N/A |
Mouse: B6.129S4-Ndufs4tm1.1Rpa/J |
Jackson Laboratory |
RRID: IMSR_JAX:027058 |
Software and Algorithms |
Isocor |
Millard et. al 2012 |
https://pypi.org/project/IsoCor/ |
GraphPad Prism Version 8 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |
Biological Samples |
hfRPE tissue |
Birth Defects Research Lab at University of Washington |
N/A |