Figure 2.

Intranasal Infusion of p75NTR Pen-siRNA Reduces Damage Following CCI. (A) Infusion of p75NTR Pen-siRNA reduced p75NTR expression in the OB and cortex as measured by Western blot. Controls received luciferase Pen-siRNA. Values represent the means ± SEM. Asterisks indicate significance by two-tailed, unpaired Student’s t test with p = .04 for OB and p = .01 for cortex. n = 3 mice/treatment. (B) Adult mice were subjected to CCI and immediately infused intranasally with either p75NTR Pen-siRNA or luciferase Pen-siRNA control. Representative images of cresyl violet-stained coronal sections from luciferase or p75NTR Pen-siRNA-treated mice marked with their coordinates to bregma. Scale bar = 1 mm. The percentage of the area of damage (region of tissue loss and penumbra, indicated by the dotted line) shows less damage in the mice that received the p75NTR Pen-siRNA. Values represent the means ± SEM. Asterisks indicate significance by two-tailed, unpaired Student’s t test with p = .03. n = 3 mice/treatment.

p75NTR = p75 neurotrophin receptor; OB = olfactory bulb.