Table 2. List of primers for the identification of diarrheagenic Escherichia coli.

Pathogena)	Primer name	Target gene	Primer sequence (5′-3′)	Amplicon (bp)	Reference
ETEC	LT-F	elt	CACACGGAGCTCCTCAGTC	508	Panchalingam et al. 2012
	LT-R		CCCCCAGCCTAGCTTAGTTT
	ST-F	est	GCTAAACCAGTAG/AGGTCTTCAAAA	147	Panchalingam et al. 2012
	ST-R		CCCGGTACAG/AGCAGGATTACAACA
EPEC	BFPA-F	bfpA	GGAAGTCAAATTCATGGGGG	367	Panchalingam et al. 2012
	BFPA-R		GGAATCAGACGCAGACTGGT
	SK1	eae	CCCGAATTCGGCACAAGCATAAGC	881	Toma et al. 2003
	SK2		CCCGGATCCGTCTCGCCAGTATTCG
STEC	VTcom-u	stx	GAGCGAAATAATTTATATGTG	518	Toma et al. 2003
	VTcom-d		TGATGATGGCAATTCAGTAT
	SK1	eae	CCCGAATTCGGCACAAGCATAAGC	881	Toma et al. 2003
	SK2		CCCGGATCCGTCTCGCCAGTATTCG
EIEC	ipaIII	ipaH	GTTCCTTGACCGCCTTTCCGATACCGTC	619	Toma et al. 2003
	ipaIV		GCCGGTCAGCCACCCTCTGAGAGTAC
	CVD432F	aatA	CTGGCGAAAGACTGTATCAT	630	Panchalingam et al. 2012
	CVD432R		CAATGTATAGAAATCCGCTGTT
EAEC	AAIC F	aaiC	ATTGTCCTCAGGCATTTCAC	215	Panchalingam et al. 2012
	AAIC R		ACGACACCCCTGATAAACAA
	aggRks1	aggR	GTATACACAAAAGAAGGAAGC	254	Toma et al. 2003
	aggRks2		ACAGAATCGTCAGCATCAGC

a) ETEC: Enterotoxigenic E. coli, EPEC: Enteropathogenic E. coli, STEC: Shiga toxin-producing E. coli, EIEC: Enteroinvasive E. coli; EAEC: Enteroaggregative E. coli.