Reagent/Resource	Source	Identifier or Catalog number
Experimental Models
U‐2 OS cells	ATCC	HTB‐96
hTERT RPE‐1 cells	ATCC	CRL‐4000
HEK 293T cells	ATCC	CRL‐3216
Recombinant DNA
Dendra2‐Lifeact7	Addgene	54694
mEmerald‐H3‐23	Addgene	54115
pH2B‐miRFP703	Addgene	80001
psPAX2	Addgene	12260
pMD.2	Addgene	12259
pLenti CMV rtTA3 Blast	Addgene	26429
Other constructs, gBlocks, etc.	This study	Table EV3
Antibodies
None	NA	NA
Oligonucleotides and sequence‐based reagents
PCR primers	This study	Table EV3
Chemicals, enzymes and other reagents
KAPA Hifi 2× polymerase	Kapa Biosystems	KK2601
Dulbecco's modified Eagle's medium (DMEM)	Thermo Fisher	11965118
DMEM, no phenol red	Thermo Fisher	21063045
DMEM/F12	Thermo Fisher	11320033
DMEM/F12, no phenol red	Thermo Fisher	21041025
Doxycycline	Sigma	D9891
Trypsin–EDTA 0.25%	Thermo Fisher	25200056
OPTIMEM	Fisher Scientific	31985070
FuGENE6	Promega	E2691
2X Gibson Assembly Master Mix	NEB	E2611L
DpnI	NEB	R0176L
DNA Clean & Concentrator	Zymo Research	D4013
GenElute HP Plasmid DNA Midiprep Kit	Sigma	NA0200‐1KT
PEG‐it Virus Precipitation Solution	SBI	LV810A‐1
Lipofectamine 3000	Thermo Fisher	L3000015
Software
Metamorph (v7.10.1.161)	Molecular Devices
Other
Leica DMi8 with Adaptive Focus	Leica
Incubation i8 chamber	Leica
TempController 2000‐1	PeCon
CO2 regulator	Oko
Spectra X Light Engine LED	Lumencor
Multi‐band dichroic filter	Spectra Services	LED‐DA‐FI‐TR‐Cy5‐4X‐A‐000
Multi‐band dichroic filter	Spectra Services	LED‐CFP/YFP/mCherry‐3X‐A‐000
Bright‐line band‐pass filter (DAPI)	Semrock	FF01‐433/24‐25
Bright‐line band‐pass filter (GFP)	Semrock	FF01‐520/35‐25
Bright‐line band‐pass filter (RFP)	Semrock	FF01‐600/37‐25
Bright‐line band‐pass filter (NIR)	Semrock	FF01‐680/42‐25
20 × 0.8 NA apochromatic objective	Leica
Mosaic 3 Digital Micromirror Device	Andor
Mosaic SS 405/1.1 W laser	Andor
iXon Ultra 888 EMCCD monochrome camera	Andor
Glass‐bottom black‐walled plates	CellVis	P06‐1.5H‐N
LSR‐II	BD Biosciences
FACS Aria III	BD Biosciences