TABLE 2.

Microbiological media used for microbial enumeration.

Growth media	Microorganisms	Method	Incubation conditions
Plate Count Agar (PCA) (Merck, Darmstadt, Germany)	Total viable count	Spread plate	30°C/72 h
De Man-Rogosa-Sharpe agar (MRS) (Oxoid, Basingstoke, United Kingdom) + natamycin 0.1%	Lactic acid bacteria	Pour plate/Overlay	30°C/72 h
Sabouraud Agar (Oxoid, Basingstoke, United Kingdom)	Yeast and molds	Spread plate	25°C/5 days
Violet Red Bile Glycose Agar (VRBGA) (BD, Sparks, MD, United States)	Enterobacteriaceae	Pour plate/overlay	37°C/24 h
Violet Red Bile Lactose Agar (VRBLA) (Oxoid, Basingstoke, United Kingdom)	Coliforms	Pour plate/Overlay	30°C/24 h
Mannitol Salt Agar (MSA) (Oxoid, Basingstoke, United Kingdom)	CoNS	Spread plate	30°C/48 h