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. 2020 Apr 8;245(9):823–834. doi: 10.1177/1535370220918193

Figure 6.

Figure 6.

Changes in the mRNA and protein expression of PKG1 in the spinal cord by H2S and inhibitors of nNOS, sGC, and PKG. Figure 6(a) shows the changes in mRNA levels of PKG1 by H2S; Figure 6(b) shows the changes in mRNA levels of PKG1 by H2S; Figure 6(c) shows the representative blots and protein level of PKG1 after rat were treated with H2S; Figure 6(d) shows the representative blots and protein level of PKG1 after rat were treated with inhibitors of nNOS, sGC, and PKG. The values are expressed as Mean ± S.E.M. H2S-L: lower concentration of H2S (10 ppm); H2S-M: middle concentration of H2S (20 ppm); H2S-H: higher concentration of H2S (40 ppm). H2S-M + 7-NI: 20 ppm H2S +10 µg 7-NI; H2S-M+ODQ: 20 ppm H2S +10 µg ODQ; H2S-M+KT5823: 20 ppm H2S-M + 500 ng KT5823. #P < 0.05 compared to DNP model; *P < 0.05 compared to H2S-M. n =12 per group.