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. 2020 Apr 23;245(9):785–796. doi: 10.1177/1535370220920832

Figure 2.

Figure 2.

High glucose activates both canonical and non-canonical NF-κB pathways in HUVEC. Cells were exposed to normal D-glucose (5 mM), high D-glucose (25 mM), or high L-glucose (25 mM) for varying durations, and cell lysates were prepared for Western blot analyses for either degradation of IκBα (the canonical pathway) or of p100 (non-canonical pathway) using specific antibodies. Signals generated by antibody to β-actin were used for normalizing the results. (a) Western blot of total lysates from endothelial cells exposed to normal D-glucose or high D-glucose for 0.5 h showing signals for IκBα and β-actin. Changes in protein levels of IκBα in cells exposed to high D-glucose are presented as ratio of IκBα/β-actin. (b) High glucose exposure induced the non-canonical NF-κB pathway by degradation of p100 subunit after 2 h of high D-glucose exposure. The graph shows reduced p100 on the Western blot after 2 h of high D-glucose exposure. Asterisks denote P < 0.05, and n= 5 to 14 in each group.