Table 2.
T and NK cell concentrations and subsets in grafts, peripheral blood in patients 28–365 days after transplantation and peripheral blood in healthy donors (HD).
| Cell subset concentration, median (IQR) | Graft, N = 88 106/kg | Day 28, N = 86 106/L | Day 56, N = 84 106/L | Day 91, N = 79 106/L | Day 180, N = 71 106/L | Day 365, N = 53 106/L | HD, N = 53 106/L |
|---|---|---|---|---|---|---|---|
| T cells (CD3) | 255 (193–330) | 420 (248–575) | 425 (260–738) | 460 (280–880) | 880 (550–1,600) | 1,200 (750–2,000) | 1,500 (1,150–1,700) |
| CD4 | 157 (109–192) | 205 (150–333) | 200 (140–328) | 230 (120–320) | 330 (220–450) | 440 (310–515) | 930 (715–1,100) |
| CD8 | 94 (66–126) | 160 (71–240) | 175 (100–395) | 220 (99–490) | 470 (210–1,000) | 690 (400–1,500) | 345 (430–630) |
| TCR γδ | 7.1 (4.5–14) | 22 (7–57) | 22 (8.8–59) | 24 (8.4–61) | 41 (13–99) | 70 (22–117) | 41 (25–81) |
| NK cells (CD16/56) | 27 (20–36) | 285 (150–403) | 200 (143–348) | 160 (100–300) | 250 (140–410) | 270 (160–380) | 200 (140–300) |
| CD56dim | 23 (16–30) | 162 (86–262) | 128 (85–215) | 112 (71–192) | 159 (100–293) | 198 (122–302) | 165 (113–250) |
| CD56bright | 1.7 (1.1–2.6) | 60 (32–98) | 34 (17–64) | 22 (13–34) | 17 (12–33) | 16 (8.8–27) | 14 (8.9–20) |
| Relative concentrations, % | |||||||
| CD4/CD3 | 60 (53–66) | 57 (46–63) | 50 (32–62) | 47 (29–57) | 37 (23–52) | 35 (24–47) | 65 (54–71) |
| CD8/CD3 | 38 (33–42) | 37 (31–50) | 43 (34–59) | 49 (34–63) | 57 (46–71) | 62 (48–75) | 32 (27–42) |
| CD4/CD8, ratio | 1.6 (1.2–2.0) | 1.6 (0.9–2.0) | 1.1 (0.6–1.9) | 1.0 (0.4–1.6) | 0.6 (0.3–1.1) | 0.6 (0.3–1.1) | 2.0 (1.3–2.7) |
| CD56dim/NK | 83 (77–88) | 59 (48–71) | 69 (57–78) | 72 (59–83) | 77 (66–84) | 80 (71–86) | 83 (78–87) |
| CD56bright/NK | 6.2 (4.3–8.5) | 22 (15–32) | 14 (10–23) | 11 (8.2–19) | 7.3 (4.6–13) | 5.7 (2.9–11) | 6.3 (4.5–9.1) |
| Naïve CD4/CD4 | 52 (44–61) | 28 (20–39) | 29 (17–38) | 29 (18–37) | 22 (14–33) | 21 (14–33) | 38 (28–49) |
| Central memory CD4/CD4 | 33 (28–40) | 41 (35–49) | 42 (34–49) | 40 (34–49) | 40 (32–48) | 44 (34–48) | 43 (36–53) |
| Effector memory CD4/CD4 | 12 (8.6–17) | 23 (17–31) | 25 (16–35) | 24 (18–35) | 29 (22–42) | 31 (21–40) | 18 (10–20) |
| TEMRA CD4/CD4 | 0.5 (0.3–1.2) | 0.8 (0.4–1.8) | 1.2 (0.6–2.4) | 1.3 (0.6–2.4) | 1.6 (1.0–5.1) | 1.7 (0.9–4.7) | 0.5 (0.2–1.8) |
| Naïve CD8/CD8 | 48 (35–61) | 26 (9.7–37) | 14 (4.9–33) | 14 (7.3–27) | 12 (6.8–22) | 10 (5.3–18) | 24 (12–37) |
| Central memory CD8/CD8 | 8.1 (5.4–11) | 5.3 (3.0–8.8) | 4.8 (2.4–9.2) | 5.6 (3.6–9.5) | 5.7 (3.1–9.8) | 5.6 (2.2–9.2) | 13 (9.8–18) |
| Effector memory CD8/CD8 | 24 (16–31) | 38 (26–51) | 39 (30–52) | 36 (27–47) | 32 (24–41) | 37 (28–52) | 31 (23–40) |
| TEMRA CD8/CD8 | 15 (10–26) | 22 (13–40) | 31 (18–44) | 35 (23–51) | 46 (33–57) | 39 (24–56) | 29 (12–42) |
| Naïve TCR γδ/TCR γδ | 7.4 (4.6–13) | 1.8 (0.9–4.0) | 1.8 (0.8–3.7) | 2.2 (0.8–4.1) | 2.1 (1.0–4.8) | 2.1 (0.7–5.2) | 3.6 (2.1–8.4) |
| Central memory TCR γδ/TCR γδ | 11 (6.9–16) | 1.1 (0.4–2.2) | 0.9 (0.4–2.7) | 0.9 (0.3–2.2) | 1.2 (0.3–3.7) | 2.0 (0.7–3.7) | 7.8 (3.9–13) |
| Effector memory TCR γδ/TCR γδ | 42 (31–54) | 48 (33–67) | 47 (30–62) | 38 (23–54) | 35 (22–50) | 44 (28–64) | 34 (23–60) |
| TEMRA TCR γδ/TCR γδ | 35 (23–50) | 46 (29–62) | 49 (35–66) | 52 (40–68) | 60 (40–73) | 47 (27–62) | 43 (25–67) |
| TCR γδ/ CD3 T | 2.7 (1.8–5.4) | 5.0 (3.0–12) | 4.9 (2.5–11) | 4.5 (2.0–10) | 3.8 (2.3–7.5) | 3.9 (2.1–7.8) | 2.7 (2.0–5.8) |
| Vδ1/ TCR γδ | 24 (11–34) | 10 (3.6–25) | 11 (3.8–21) | 12 (3.2–27) | 21 (9.0–42) | 31 (11–51) | 26 (12–42) |
| Vδ2/ TCR γδ | 68 (50–83) | 87 (69–95) | 86 (68–95) | 84 (64–96) | 71 (41–90) | 61 (34–85) | 65 (39–83) |
| NonVδ1–non-Vδ2/ TCR γδ | 6.1 (2.5–9.9) | 2.0 (1.0–5.8) | 2.1 (0.7–5.1) | 2.7 (0.9–42) | 3.5 (0.9–9.0) | 3.9 (1.2–12) | 6.0 (2.5–16) |