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. Author manuscript; available in PMC: 2020 Jun 5.
Published in final edited form as: Free Radic Biol Med. 2019 Nov 9;146:287–298. doi: 10.1016/j.freeradbiomed.2019.11.012

Figure 4. Mitochondrial CaMKII inhibition prevents ROS production and apoptosis.

Figure 4.

(A, B) Confocal microscopy of mitoSox fluorescence at 24 h after irradiation with 4 Gy, adjusted for mitoTracker fluorescence in BAECs (A, B) and HMVECs (C) transduced with adenovirus expressing mitoCaMKII or control for 48h prior to irradiation. Control measurements were taken at the same time points in cells without radiation exposure. n= 75 cells per conditions. (D) Time course of mitochondrial ROS production in BAECs transduced with adenovirus expressing mitoCaMKII or control by FACS after irradiation with 4 Gy or no radiation exposure. n = 4 independent experiments. (E) Time course of cytosolic ROS production after irradiation with 4 Gy or no radiation exposure in BAECs transfected with Cyto-RoGFP cDNA, followed by infection with adenovirus expressing mitoCaMKII or control. n = 4 independent experiments. (F) Annexin V fluorescence at 24 h after irradiation in BAECs treated as in (A, B) by FACS. n = 4 independent experiments. (A-D, F) * p<0.05 versus unirradiated control cells. # p < 0.05 versus irradiated control cells. (E) * p < 0.05 versus 0 h, @ p < 0.05 4 h versus 24 h, # p , 0.05 control versus mitoCaMKIIN, ^ p < 0.05 IR versus no IR.