Fig. 3. Natural hemozoin qualitatively enhances expression of NOD2.
An indirect immunofluorescence staining was applied in immortalized BMDMs primed with IFN-γ for 1–2 h and treated for 24 h with Medium, MDP (2.5 μg/ml), or nHZ (30 μM). Cells were probed with NOD2 polyclonal antibody and Alexa Fluor® 488 goat anti-rabbit antibody (FITC) (see methodology section). The figures are one representative experiment out of three independent tests.