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. Author manuscript; available in PMC: 2021 Feb 14.
Published in final edited form as: Circ Res. 2019 Dec 9;126(4):517–529. doi: 10.1161/CIRCRESAHA.119.315968

Figure 6. TCF21 blocks transcriptional regulation by MYOCD at the SRF enhancer, the TAGLN promoter and the CNN1 promoter.

Figure 6.

A, B) Either one or both MYOCD (pcDNA3.1-MYOCD) and TCF21 (pCMV6-AC-TCF21) expression plasmids were co-transfected with a reporter construct containing the SRF enhancer (SRF-luc) into A7r5 (A) and HCASMC (B). Dual luciferase assays were performed, using Renilla luciferase reporter plasmid as an internal control of transfection efficiency. C) Dual luciferase assays were conducted using a reporter construct containing the TAGLN promoter (TAGLN-luc) transfected into A7r5cells, and D) HCASMC with the same methodology. E) Dual luciferase assays were conducted using a reporter construct containing the CNN1 promoter (CNN1-luc) transfected into A7r5cells, and F) HCASMC with the same methodology. All experiments, (n>8). Each group was compared to “TCF21(−) with MYOCD” (A,C,E) or “MYOCD” (B,D,F) using one-way ANOVA followed by Dunnett’s post-test (B,D-F), or Kruskal-Wallis test, followed by Dunn’s post-test (A,C).