Skip to main content
. Author manuscript; available in PMC: 2020 Aug 24.
Published in final edited form as: Nat Biotechnol. 2020 Feb 24;38(4):460–470. doi: 10.1038/s41587-020-0430-6

Figure 2. Single-cell RNA sequencing demonstrates that cytoskeletal state directs pancreatic progenitor fate.

Figure 2.

(a-b) tSNE projections of single-cell RNA sequencing performed on plated stage 4 cells either untreated (n = 286 cells), treated with 0.5 μM latrunculin A (n = 579 cells), or treated with 5 μM nocodazole (n = 197 cells). Unsupervised clustering of the combined cell population from all three conditions revealed five separate clusters. (c) Violin plots indicating the relative expression of important pancreatic genes in each cluster. (d) The percentage of cells from the indicated treatment condition that were within each of the five clusters. (e) Violin plot of NKX6–1 expression within the pancreatic progenitor cluster. All data was generated with HUES8.