Fig 6. A HFD alters the microbial composition in w¹¹¹⁸ flies.

(A) Principal coordinate analysis showing significant alterations in the intestinal microbiota triggered by a CD or HFD. Each data point corresponds to one biological replicate. (B) Linear discriminant analysis effect size (LEfSe) to determine differentially enriched bacteria in the intestinal microbiota of flies fed a CD or HFD. (C–F) Fecal transplantation into the esg-Gal4::UAS-GFP reporter strain was performed to examine the effect of diet-induced changes in the microbial composition on intestinal cell proliferation. Flies were reconstituted with a microbiota derived from flies fed a CD (C–D) or HFD (E–F). Samples were analyzed after 3 days (C and E) or 5 days (D and F). (G) Quantitative analysis of the data presented in (C–F) (n = 8–12). CD = control diet, HFD = high-fat diet.