Fig 5. Cytokine secretion from human PBMCs is mediated by activation of NFκB and p38 MAPK.

PBMCs were treated with indicated inhibitors 2 h prior to infection and subsequently infected for 20 h, after which IL12/IL23p40 (a), IL1β (b) and IFNγ were measured. The relative parasite growth was measured by luciferase growth assay (d). Each dot represents the mean of 3 technical replicates from an experiment. Statistical analysis was done with One-way ANOVA followed by Tukey’s multiple comparison test. Data are represented as mean ± standard error of the mean (SEM).