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. 2020 May 26;18(5):e3000660. doi: 10.1371/journal.pbio.3000660

Fig 4. Expression of APP RNA by in situ hybridization in the FCs of SIV-infectedmacaques and patients who are infected with HIV.

Fig 4

Representative FISH and IF photomicrographs showing differential expression of APP RNA in GFAP+ astrocytes in SIV+ macaques (A) and patients who are HIV positive (B). Scale bar, 10 μm. Quantitative analysis of percent GFAP+ astrocytes colocalized with APP RNA in saline and SIV+ (C) and patients who are HIV positive (D). Ten fields from FC/macaque/patient were analyzed from n = 3 macaques and n = 3 patients. Data are presented as mean ± SEM. Student t test was used to determine the statistical significance: *P < 0.05 versus saline/control. Arrows indicate GFAP-positive astrocytes colocalized with APP RNA. The data underlying this figure may be found in S4 Data. APP, amyloid precursor protein; FC, frontal cortex; GFAP, glial fibrillary acidic protein; SIV, simian immunodeficiency virus.