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. 2020 May 26;14:2079–2090. doi: 10.2147/DDDT.S244059

Figure 1.

Figure 1

Resveratrol down-regulated TLR4/NF-кB expression via MyD88-dependent and -independent signaling pathway in IL-1β-induced SW1353 cells. (A) SW1353 cells were transfected with TLR4 siRNA (100 nM) or control siRNA for 48 h, TLR4 expression was analyzed by Western blot. (B) The levels of TLR4 were normalized with β-actin. The results for Western blot were expressed as folds of CON. *P <0.05, versus the CON, #P <0.05 versus control siRNA. (C) SW1353 cells were transfected with TLR4 siRNA (100 nM) or control siRNA for 48 h and then exposed to 10 ng/mL IL-1β with or without 50 μM resveratrol (RES) for 24 h, TLR4, MyD88, TRIF, and p-NF-κB p65 expression were analyzed by Western blot. (D–G) The levels of TLR4, MyD88, TRIF and p-NF-κB p65 were normalized with β-actin. The results for Western blot were expressed as folds of CON. All data were expressed as the mean ± SD of three independent experiments. **P <0.01 versus the CON, #P <0.05, ##P <0.01 versus the IL-1β, $$P <0.01 versus IL-1β + RES, &&P <0.01 versus siRNA, ^^P <0.01 versus siRNA + IL-1β.