This work is licensed under a Figure 3.
miR-23b-5p expression was differentially regulated in the activated and the aging BAT. (A) miR-23b-5p expression in BAT and WAT between room temperature (RT) and cold stimulation (4°C) groups were evaluated by real-time PCR (n = 12 per group). (B) miR-23b-5p expression in BAT and WAT of mice intraperitoneally administered with β3-adrenergic agonist CL-316,243 (1 mg/kg body weight) were detected by real-time PCR (n = 12 per group). (C) Relative abundance of miR-23b-5p in BAT of age group 1-month- (1M), 2-month- (2M), 5-month- (5M), 8-month- (8M), and 10-month-old (10M) male C57BL6 mice was determined by real-time PCR (n = 6 per time point). (D, E, and F) Correlation between the mRNA expression of miR-23b-5p and Ucp1 in the activated (upper and middle) and aging (lower) BAT. Ppia or U6 expression was used as internal control for mRNA or miRNA expression analysis respectively. Values are the means ± s.d. *P < 0.05; **P < 0.01; ***P < 0.001.