Table 1. Cryo-EM data collection and structure refinement statistics.
| Cryo-EM structure of the StOAD βγ sub-complex (PDB 6IWW) | |
|---|---|
| Data collection and procession | |
| Magnification (nominal) | 105,000 |
| Voltage (kV) | 300 |
| Electron exposure (e-/Å2) | 50 (32 frames) |
| Defocus range (μm) | 1.0–3.5 |
| Pixel size (Å) | 0.68 |
| Symmetry imposed | C3 |
| Initial particle images (no.) | 260,982 |
| Final particle images (no.) | 75,699 |
| Map resolution (Å) | 3.88 |
| FSC threshold | 0.143 |
| Refinement | |
| Initial model used | EMBUILDER generated model |
| Model resolution (Å) | 3.88 |
| FSC threshold | 0.143 |
| Map sharpening B factor (Å2) | −250 |
| Model composition | |
| Non-hydrogen atoms | 10,227 |
| Protein residues | 1386 |
| Ligands | 3 |
| B-factors (Å2) | |
| Protein | 41.7 |
| Ligand | 23.9 |
| R.m.s deviations | |
| Bond lengths (Å) | 0.008 |
| Bond angles (°) | 1.016 |
| Validation | |
| Molprobity score | 1.61 |
| Clashscore | 5.83 |
| Poor rotamers (%) | 0 |
| Ramachandran plot | |
| Favored | 95.78 |
| Allowed (%) | 4.22 |
| Disallowed (%) | 0 |