Figure 4. Super-functional T cells exhibit Th1 characteristics and are CD40L^hi ICOS⁺.

(A) Bin plots visualize the co-expression of PD-1 and IFN-γ with various proteins in stimulated (PMA/ionomycin) splenic T cells of old mice. (B) Distribution of the top 50% Z⁺ cells of selected markers from (A). If a bin contains the minimum number of cells (10), the frequency of the third marker is shown in pseudo-colors. Cell frequencies per quadrant are calculated on the number of Z⁺ cells in the quadrant (red) and number of Z⁺ cells per sample (green). Data are representative for at least two independent experiments with n ≥ 3 mice.

Figure 4—figure supplement 1. Super-functional T cells are CD40L^hi ICOS⁺.

(A) Bin plots visualize the relative expression level of parameter Z (MFI+) per bin of PMA/ionomycin stimulated splenic T cells. Grey bins contain less than 10 Z⁺ cells. (B) Histograms as example stainings for Z parameters. Data are representative for at least two independent experiments with n ≥ 3 mice.