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Identification of AvPAL* mutants (–) by growth-coupled enrichment. (a.) Purified PAL activity of 15 randomly picked colonies compared to wildtype. (b.) The growth profiles (solid) and tCA production (dotted) in MMphe,opt of select mutants (○ and △). (*** significant compared to wildtype or silent mutants (✕), p < 0.001)
