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. Author manuscript; available in PMC: 2021 Apr 1.

Published in final edited form as: Adv Healthc Mater. 2020 Jan 20;9(8):e1901214. doi: 10.1002/adhm.201901214

Figure 3. — a) ISC colony survival is dependent on macromer wt%, and therefore modulus, with the highest viability seen at 5 wt% and 1.3 kPa. b) ISC colonies were marked as viable if they maintained stemness, as marked by GFP expression, and were polarized, as visualized by F-Actin staining. Scale bar 100 μm. Data is represented as means +/− standard deviation. At least three hydrogels were analyzed per condition and data was analyzed using a one-way Anova with multiple comparisons. *p<0.05, **p<0.01, ***p<0.001

Figure 3. — a) ISC colony survival is dependent on macromer wt%, and therefore modulus, with the highest viability seen at 5 wt% and 1.3 kPa. b) ISC colonies were marked as viable if they maintained stemness, as marked by GFP expression, and were polarized, as visualized by F-Actin staining. Scale bar 100 μm. Data is represented as means +/− standard deviation. At least three hydrogels were analyzed per condition and data was analyzed using a one-way Anova with multiple comparisons. *p<0.05, **p<0.01, ***p<0.001