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. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Angew Chem Int Ed Engl. 2020 Mar 17;59(21):8108–8112. doi: 10.1002/anie.201916712

Figure 1.

Figure 1.

(A) UV-Vis spectra of 4 μM hemin (dotted trace), mixture of 30 μM Ac-LHLHLFL-NH2 with 4 μM hemin (dashed trace) and 30 μM Ac-LILHLFL-NH2 with 4 μM hemin (solid trace). Inset: EPR spectrum of 750 μM Ac-LILHLFL-NH2 with 100 μM hemin (B) Circular Dichroism spectra of 30 μM Ac-LHLHLFL-NH2 (dashed trace) and 30 μM Ac-LILHLFL-NH2 (solid trace). Inset: CD of Soret band of LHLHLFL-NH2 with 4 μM hemin (dashed trace) and 30 μM Ac-LILHLFL-NH2 with 4 μM hemin (solid trace). All measurements were taken in 100 mM phosphate buffer at pH 7 after 24 hours of incubation.