. Author manuscript; available in PMC: 2021 May 18.

Published in final edited form as: Angew Chem Int Ed Engl. 2020 Mar 17;59(21):8108–8112. doi: 10.1002/anie.201916712

Table 3.

Kinetic parameters for cyclopropanation promoted by hemin-bound self-assembled peptides and representative engineered heme proteins.

Catalyst	k_cat, s⁻¹	K_M(EDA), mM	K_M(Styrene), mM	Ref.
LILHLFL - hemin^[a]	0.905 ± 0.068	2.96 ± 0.55	2.76 ± 0.42	this work
LALHLFL-hemin^[a]	0.600 ± 0.004	3.06 ± 0.45	2.01 ± 0.26	this work
P450_BM3-heme-CIS^[b]	1.7 ± 0.4	5.2 ± 3.5	1.4 ± 0.5	[13]
Mb H64V/ V68A^[c]	-	5	2	[11a]

^[a]

Reaction conditions: 20 μM hemin with 150 μM peptide, 1 − 8 mM 4-(trifluoromethyl)styrene, 2.5 − 24 mM EDA, 10 mM dithionite, 100 mM pH 7 phosphate buffer.

^[b]

Reaction was performed at pH 8 with 0.2-20 mM EDA and 30 mM styrene or 0.2-15 mM styrene and 20 mM EDA

^[c]

Reaction was performed at pH 7 with styrene as substrate.