Skip to main content
. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Adv Healthc Mater. 2019 Dec 23;9(8):e1901228. doi: 10.1002/adhm.201901228

Figure 3.

Figure 3.

MFC behavior on thick layers of soft and stiff fibrous networks. (a) Schematic of PDMS wells containing suspended thick fibrous networks, viewed from the (i) top or (ii) side. MFCs were seeded on top of the PDMS wells. (b) Quantification of thickness of hydrated fibrous networks after 24 hours of swelling. NS: not significant. Representative (c) 3D reconstructed and (d) cross-section images of MFCs cultured on soft and stiff fibrous networks (green) for 1 or 24 h. Cells were stained for nuclei (blue) and F-actin (red). Scale bars are (c) 10 μm and (d) 20 μm. White arrows and dotted line in d indicate approximate location of MFCs and top surface of fibrous networks, respectively. (e) Quantification of percentage of MFCs within fibrous networks. n = 4 wells. Two-way ANOVA with Tukey’s post hoc testing. ***p < 0.001.