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. Author manuscript; available in PMC: 2021 Jun 5.
Published in final edited form as: Circ Res. 2020 Mar 20;126(12):1779–1794. doi: 10.1161/CIRCRESAHA.120.316659

Figure 3. Deletion of integrin α9 in myeloid cells improves local cerebral blood flow and inhibits post-ischemia/reperfusion thrombosis.

Figure 3.

A. Left: Representative images were taken using laser speckle imaging of regional cerebral blood flow (CBF) in the cortical region. Right: Quantification at different time points (5–90 minutes). n=6, 5. B. Brain homogenates from the infarcted and peri-infarcted area following 1-hour ischemia/23 hours reperfusion was processed for Western blotting: Representative Western blots and densitometric analysis of fibrin(ogen) and platelets (CD4-positive). β-Actin was used as a loading control. n=4/group. C. Left: Representative immunostaining images for platelet (CD41-positive, green) and fibrin(ogen) (red). Scale bar: 100 μm. Right: Thrombotic index, n=4/group D. Left: Representative microphotographs depicting percentage occlusion ∼12 minutes after FeCl3-injured carotid arteries as visualized by upright intravital microscopy. Platelets were labeled with calcein green. White lines delineate the arteries. Right: Mean time to complete occlusion of FeCl3 injured carotid artery, n=8, 8. Data are mean ± SEM. Statistical analysis: Two-way ANOVA followed by Holm-Sidak multiple comparisons test (3A), unpaired t-test (3B, 3C 3D, and 4D).