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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2020 Mar 3;200:105650. doi: 10.1016/j.jsbmb.2020.105650

Figure 2: CYP27B1 is detected in myofibers in ALS but not normal control muscle.

Figure 2:

Sections from vastus lateralis muscle were immunostained with a CYP27B1 antibody and counterstained with hematoxylin. (A) Low power view of an ALS muscle section shows positive immunostaining (brown color) of myofibers. (B) High-powered view of boxed area in (A) shows myofiber staining and surrounding mononuclear infiltrate. Here the muscle fibers range between 5-50 μM in diameter. One group of atrophic angular fibers contains up to 20 muscle fibers. (C) Muscle section from a normal control shows no immunostaining. (D) Muscle section from another ALS patient again shows immunostaining of some, but not all, myofibers. Likewise, there is an increase in mononuclear cells surrounding some of the fibers. (E) A second normal control muscle section shows no immunostaining. Scale bars, 50 μM.