Skip to main content
. Author manuscript; available in PMC: 2021 Jun 3.
Published in final edited form as: Neuron. 2020 Mar 25;106(5):842–854.e4. doi: 10.1016/j.neuron.2020.03.002

Figure 6. Mechanisms of input-specific heterosynaptic plasticity.

Figure 6.

(A) Thapsigargin in whole-cell pipette (10 μM) prevented heterosynaptic excitatory and inhibitory LTD after pre→post pairing. Top, excitatory inhibitory LTP induced by pre→post pairing at channel S4 (red, Δt=4 msec). Middle, thapsigargin prevented heterosynaptic LTD. Bottom, Rs and Ri.

(B) Spike pairing with normal solutions and ACSF on paired (red), original best unpaired (blue) and other unpaired inputs (black); same recordings as Figures 2A,B. Filled bars, excitation; open bars, inhibition.

(C) Blocking NMDA receptors (50 μm APV in bath) prevented plasticity (pre→post n=6: paired EPSCs p>0.7, Student’s paired two-tailed t-test, paired IPSCs p>0.4, originally-largest unpaired EPSCs p>0.6, originally-largest unpaired IPSCs p>0.5; post→pre n=4: paired EPSCs p>0.8, paired IPSCs p>0.7, originally-largest unpaired EPSCs p>0.6, originally-largest unpaired IPSCs p>0.5).

(D) Intracellular ruthenium red (20 μm) spared homosynaptic but prevented heterosynaptic plasticity at original best inputs (pre→post n=9: paired EPSCs p<0.02, paired IPSCs p<0.05, originally-largest unpaired EPSCs p>0.6, originally-largest unpaired IPSCs p>0.3, post→pre n=9: paired EPSCs p<0.004, paired IPSCs p<0.006, originally-largest unpaired EPSCs p>0.9, originally-largest unpaired IPSCs p>0.6).

(E) Intracellular thapsigargin (10 μm) spared homosynaptic but prevented heterosynaptic plasticity (pre→post n=12: paired EPSCs p<0.006, paired IPSCs p<0.003, originally-largest unpaired EPSCs p>0.05, originally-largest unpaired IPSCs p>0.1; post→pre n=10: paired EPSCs p<0.01, paired IPSCs p<0.006, originally-largest unpaired EPSCs p>0.05, originally-largest unpaired IPSCs p>0.2).