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. 2020 Jun 5;11:2861. doi: 10.1038/s41467-020-16641-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Coverage plots for the B1 tumor sample for the two fusion partners KMT2A (targeted) and MLLT6. b Coverage plots for the B2 tumor sample for the two fusion partners BCR (targeted) and ABL1. BCR was targeted with eleven sequential guides to span the possible breakpoint region. Dotted lines (green) indicate the crRNA-directed Cas9 cleavage positions and dashed lines (red) indicate breakpoint positions. Arrows indicate the directionality of reads created from the specific crRNA design. c Mean coverage and d mean enrichment across the genome, on-target (from cut to breakpoint), BP-spanning (breakpoint-spanning), and fusion-spanning (across the fusion junction) for the tumor samples B1 and B2. e Time-course experiment on sequencing time necessary to identify fusion-spanning reads (%) and f fusion-spanning reads (n = 2). Source data are provided as a Source Data file.