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. 2020 Jun 5;11:2856. doi: 10.1038/s41467-020-16625-7

Fig. 6. IFN-β elevates IL-6 and exacerbates TH17-driven EAE.

Fig. 6

MOG-primed TH17 cells were transferred into recipient mice and treated with 1000 U of IFN-β or vehicle every other day from day 0–10 post transfer of cells. a Clinical scores of mice with TH17-EAE treated with vehicle or IFN-β; (TH17-vehicle n = 5, TH17-IFN-β n = 5) and two-tailed Mann–Whitney tests were performed to determine statistical significance (*P < 0.05). Representative of four experiments with similar results. b Spinal cord sections from mice (day 30) were stained with H&E and Luxol fast blue. Representative images of four mice in each group. Scale bar represents 100 µm. c Levels of IL-6 in the sera (measured by ELISA) of TH17-EAE (day 2) were elevated with IFN-β; (TH17-vehicle n = 5, TH17-IFN-β n = 5, healthy n = 3) and a two-tailed Kruskal–Wallis test with multiple comparisons corrected by the Dunn’s method was used to determine statistical significance. d Representative flow cytometry plots and absolute numbers of live CD4+ T cells in the spinal cords of EAE mice, 30 days post transfer. e Representative flow cytometry plots and absolute numbers of CD4+ T cells expressing IL-17, GM-CSF or both in the spinal cords of EAE mice. f Representative flow cytometry plots and absolute numbers of live CD19+ B cells in the spinal cords of EAE mice. (TH17-vehicle n = 5, TH17-IFN-β n = 5) and two-tailed Mann–Whitney tests were performed to determine statistical significance. Error bars indicate the S.E.M. Source data are provided as a Source Data file.