Fig. 4.
MACC1 does not affect integrin distribution. a, b Distribution of integrin β1 and α5 relative to the cell boundary for U138/EV and U138/MACC1 cells, plated on glass, fibronectin and laminin. No significant change in distribution could be observed. The inlet in a) displays an U138 cell labeled with DAPI (nucleus), phalloidin (actin) and an integrin β1 antibody. The red closed line shows the boundary and the red dot in the cell depicts the center of the nucleus. c, d Distribution of integrin β1 and α5 relative to the cell boundary for U251/EV and U251/MACC1 cells, plated on glass, fibronectin and laminin. No significant change in distribution could be observed. The following numbers of images of three independent experiments were analyzed: nU138/EV Glass = 17, nU138/EV FN = 34, nU138/EV LN = 52, nU138/MACC1 Glass = 30, nU138/MACC1 FN = 44, nU138/MACC1 LN = 38, nU251/EV Glass = 35, nU251/EV FN = 67, nU251/EV LN = 70, nU251/MACC1 Glass = 36, nU251/MACC1 FN = 77 and nU251/MACC1 LN = 55