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. 2020 Jun 5;20:521. doi: 10.1186/s12885-020-06969-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Characterisation of the OCT4-positive cell population in HCC827 and HCC827 GRH2 cells. a Representative bright-field images of wound-healing experiments acquired at 0- and 12-h post-wounding. Scale bar: 100 μm. b Migration rate was expressed as the percentage of wound-closure area. c Detection of possible OCT4 translation in HCC827 and HCC827 GRH2 cells using the FLAG-tagged genomic transgene (pOCT4Gen-FLAG) according to immunocytochemistry. RED: transfected RFP-positive cells; GREEN: FLAG-positive cells. The ratio of FLAG−/RFP-positive cells for each is indicated. Scale bar: 100 μm. d–h Effects of ablating OCT4-positive cells from HCC827 GRH2 cells. d Phase-contrast image of pOCT4-EGFP- or pOCT4-DTA-transfected HCC827 GRH2 cells at 24-h post-transfection. These cells were used for the assays described in e–h. e OCT4-positive cell rate among pOCT4-DTA-transfected HCC827 GRH2 cells. f Sphere-formation activities, g cell migration according to Transwell migration assays, and hSPP1C mRNA levels by qPCR. Data represent the mean ± standard deviation from three independent experiments. *P < 0.05, **P < 0.005, ***P < 0.0005, compared with the control. Scale bar: 100 μm