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. 2020 Jun 5;20:217. doi: 10.1186/s12935-020-01312-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — F-actin distribution, amounts, and depolymerization. a Distribution of F-actin in four different cells, observed by optical (left column) and fluorescence (right column) microscopy. b Western blot analysis of soluble actin (G-actin) and insoluble actin (F-actin). The ratio of F-actin to total actin (G- and F-actin). c Morphological change in cells induced by the LatA treatment. The same position on the cell was indicated with an arrow to compare morphological changes according to the treatment time. d Relative cell area reduction as a function of LatA treatment time. Each value represents mean ± SEM (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001)