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. 2020 Jun 5;20:258. doi: 10.1186/s12870-020-02451-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Functional analysis of the IbMYB340, IbbHLH2 and IbNAC56a or IbNAC56b genes using a transient expression assay in tobacco leaves. a The phenotype of tobacco leaves on the 7th day after infiltration: a, pSAK277; b, IbNAC56a; c, IbNAC56b; d, IbNAC56a + IbNAC56b; e, IbbHLH2; f, IbMYB340; g, IbMYB340 + IbNAC56a; h, IbMYB340 + IbNAC56b; i, IbMYB340 + IbbHLH2; j, IbMYB340 + IbbHLH2 + IbNAC56a; and k, IbMYB340 + IbbHLH2 + IbNAC56b. Scale bar = 1 cm. b Total anthocyanin contents in transformed leaves of tobacco. FW, fresh weight. c-d The color parameter L* and a*/b* ratio values were measured with a Minolta Chroma Meter and were used to indicate phenotypic changes. The values are presented as the means ± SDs (n = 3), and the uppercase and lowercase letters represent significant differences at P < 0.01 or P < 0.05, respectively