Fig. 2.

Monitoring the spread of the disease and the measurement of parasite burden in the footpad and ear injected with different lines of L. tropica (L. tropica^WT, L. tropica^EGFP, and L. tropica^EGFP-LUC). (A) Measuring the infected footpad’s thickness by a caliper in groups G1, G2, and G3; (B) measuring the diameter of inflammation in the infected ear by a ruler in G4, G5, and G6; (C) parasite load in the popliteal dLNs in G1, G2, and G3; (D) parasite burden in the retromaxillar dLNs in G4, G5, and G6 assessed through the limiting dilution method at four time points (1, 2, 3, and 6 months after the start of the infection). The bars indicate the SD of the mice or dLNs (4-5 individual mice in each group). ^*p < 0.05