Fig. 1.

TDF and TAF inhibit EBV lytic DNA replication. (A) HH514-16 cells were induced with 3 mM butyrate for 96 h and simultaneously treated with the established anti-herpesvirus drugs ACV and GCV or the TFV prodrugs TDF and TAF. EBV copies per genome were measured by qPCR after 96 h of treatment at a 50 μM dose. Results were analyzed from three independent experiments; each value shown. One-way ANOVA (P < 0.05) was followed by multiple hypothesis testing between butyrate and each condition. Statistical significance is highlighted by P values as follows: **P < 0.01. (B) Viability was measured by trypan blue exclusion after 96 h. Results were analyzed from four independent experiments; each value shown. One-way ANOVA (P < 0.05) was followed by multiple hypothesis testing between butyrate and each condition. Statistical significance is highlighted by P values as follows: *P < 0.05; ***P < 0.001.