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Atg7 defective cells were generated by inhibiting gene expression of the Atg7 gene with RNA interference. The cells in which Atg7 was silenced show no staining with anti-ATG7, while wild type cells do. This type of validation was marked as validation by negative control (RNA interference) and by genetic strategies of the five pillars. From Abu-Remaileh et al. (2017). Reprinted with permission from AAAS.
