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. 2020 Jun 3;8:e9281. doi: 10.7717/peerj.9281

Figure 2. The combination treatment activates apoptosis pathway and inhibits IRAK1/NF-κB, ERK pathways in MB231-PR cells.

Figure 2

(A) Western blot analysis of proteins expression after cells treated with DMSO, 75 nM PTX, 10 μM GPT and different combination for 24 h. (B) Western blot analysis of proteins expression after cells treated with DMSO, 75 nM PTX, 10 μM GPT and combination for 24 h and 48 h, respectively. (C–H) qPCR analysis of IRAK1/NF-κB downstream inflammatory cytokines and S100A7/9 gene expression after cells treated for 24 h and 48 h, respectively. *P < 0.05, **P < 0.01, ***P < 0.001. P-values were calculated with t test.