Fig. 2.

Hop2-Mnd1 stimulates efficient strand exchange by bypassing the donor dsDNA end dependency. (A) dsDNA ends examined in the strand exchange reactions in B. (B) Strand exchange reactions were conducted with lds with various ends as indicated. css was first incubated with Dmc1 for 5 min, then supplemented with Swi5-Sfr1 or Hop2-Mnd1 and RPA, and incubated for a further 5 min. The reaction was then initiated through the addition of lds and incubated for 1 h at 30 °C. Reaction products were analyzed by agarose gel electrophoresis. css, 10 µM; Dmc1, 5 µM; Hop2-Mnd1, 0.25 µM; lds, 10 µM; RPA, 1 µM; Swi5-Sfr1, 0.5 µM. Mean values ± SD from three independent experiments are shown.