Fig. 2.

CPSFL1 encodes a chloroplast-targeted CRAL-TRIO domain protein that binds PA. (A) Phylogenetic analysis of A. thaliana and C. reinhardtii CRAL-TRIO proteins. The gene At5g63060 encodes the A. thaliana chloroplast-localized ortholog of C. reinhardtii CPSFL1 (colored in green). Genes with an additional Golgi dynamics domain or nodulin domain are color-coded in orange and blue, respectively. (B) CPSFL1 protein lipid overlay assay. Stained Coomassie gel of purified CPSFL1 protein is shown on the left. Lipid abbreviations are detailed in Methods. (C) Fluorescently labeled CPSFL1 proteins bind to GUVs containing 30% DOPA. (Scale bar, 10 μm.) (D) CPSFL1 fluorescence intensity quantification on GUV membranes (Right). Data represent means ± SE. Asterisk (***) marks significant difference relative to the 0% PA GUVs at P < 0.001, derived using a two-sample Student’s t test.