Fig. 4.
The cpsfl1 mutant shows reduced carotenoid content. HPLC pigment analysis of carotenoids (A) and chlorophylls (B) of dark-grown WT, cpsfl1, and cpsfl1(CPSFL1) complemented cells. (C) Growth phenotypes and PSII activity (Fv/Fm) of chlL and chlL cpsfl1 double mutant. Cells were spotted onto minimal (HSM) and acetate-containing (TAP) solid media and grown under indicated light intensities. The term “μE” represents micromole photons m−2 s−1. (D) HPLC pigment analysis of chlL and chlL cpsfl1 cells grown in the dark. Error bars represent means ± SD (n = 3). Significantly changed pigments relative to the WT (two-tailed Student’s t test; P < 0.05) are marked with asterisks. Abbreviations: α-Car, α-Carotene; β-Car, β-Carotene; Ant, Antheraxanthin; Chl, Chlorophyll; Lor, Loroxanthin; Lut, Lutein; Neo, Neoxanthin; Vio, Violaxanthin; Zea, Zeaxanthin.