Fig. 2.

SINV infection induces TE modulation in D. simulans Makindu flies. (A and F) TE transcript modulation upon SINV infection, expressed as log₂ of the ratio (counts in infected condition/counts in mock condition) for each annotated TE family, in carcasses and ovaries, respectively. TE-derived 23- to 30-nt small RNA modulation upon SINV infection, in carcasses and ovaries, respectively (B and G), and TE-derived 21-nt small RNA modulation upon SINV infection, in carcasses and ovaries, respectively (C and H). Small RNA amounts were normalized relative to miRNAs. (D) TE transcript levels, as assessed by RT-qPCR, are expressed as fold changes relative to rp49 transcript levels. The majority of TE families show a reduction of transcript amounts upon infection. Error bars are SDs. (E) Ping-pong signatures for 23- to 30-nt RNAs in Makindu carcasses. Significant enrichment in 10-nt overlaps (i.e., ping-pong signatures) could be observed, as illustrated here by the 412 and doc families.