Fig. 2.
The putative gene cluster for momilactone biosynthesis in C. plumiforme. (A) The genomic synteny of the BGCs for momilactone formation in plants. (B) Time course gene-expression profiles of the four genes (CpDTC1/HpDTC1, CpMAS, CpCYP970A14, and CpCYP964A1) found in the momilactone BGC after the treatment of CuCl2 or chitosan. CpMAS: Unigene_5051, LC494432; CpCYP970A14: Unigene_16484, LC494433; CpDTC1/HpDTC1: LC128408; CpCYP964A1: Unigene_12783, LC494434. The data shown were normalized by comparison to the expression of the housekeeping gene CpACT3. (C) Comparison of basal expression levels of the four genes in the cluster. The expression data represent the mean ± SD of three biological replicates with independent elicitor treatments to C. plumiforme gametophytes.