Fig. 1.

The ability to respire is crucial for survival and energy maintenance upon acute glucose starvation. (A) Relative ATP levels in the respiratory-deficient cbp2Δ mutant and wild-type cells treated with the respiration inhibitor Antimycin A, compared to wild type after 1 and 19 h of acute glucose starvation. Mean, SD, and biological replicates are shown. (Right) Survival after 1 h, 3 d, and 6 d of acute starvation for wild-type (WT), cbp2Δ mutants, and wild-type cells treated with Antimycin A (WT + AntA) during starvation. (B) Relative change in energy charges during starvation. Change in ion intensity for ATP, ADP, and AMP after 0, 1, 2, 3, and 4 h of acute glucose starvation (red, glucose starved), compared to nonstarved cells (blue, nonstarved). Comparison between WT and cbp2Δ cells. Average and SE (error bar) of two biological replicates are shown. (C) Change in ion intensity for metabolites after 0, 1, 2, 3, and 4 h of acute glucose starvation (red, glucose starved), compared to nonstarved cells (blue, nonstarved). Comparison between WT cells and cbp2Δ cells. FBP, fructose bisphosphate; G6P, glucose-6-phosphate; Glycerol P, Glycerol phosphate; GMP, guanosine monophosphate; Ms1P, mannose 1-phosphate; R5P, ribose-5-phosphate; S7P, sedoheptulose-7-guanine; PEP, phosphoenolpyruvate. Average and SE (error bar) of two biological replicates are shown.