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. 2020 May 19;117(22):12239–12248. doi: 10.1073/pnas.1913370117

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Fig. 4. — Unlike proteasomal activity, bulk autophagy contributes to survival and energy maintenance upon acute glucose starvation. (A) ATP levels after 1 h of pretreatment with MG132 or DMSO as a control, followed by 1 h in acute SC or SCD medium with MG132/DMSO only. (B) ATP levels in cbp2Δ and atg2Δ relative to WT cells after 1 and 19 h of acute glucose starvation. (C) Survival of atg2Δ cells after 2 h to 14 d of glucose starvation. Means, SDs, and biological replicate values are shown. Significances are shown between the wild-type and mutants (ns, indicates nonsignificant; *0.05 > P > 0.01; **0.01 > P > 0.001; ***0.001 > P > 0.0001; and ****P < 0.0001). Significance was calculated using a one-way ANOVA test followed by a Holm-Sidak test.