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. 2020 May 19;117(22):12341–12351. doi: 10.1073/pnas.2005156117

Fig. 2.

Fig. 2.

KLF7 loss inhibits PDAC tumor growth and metastasis. (A) Representative images of soft agar colonies of PDAC cell lines expressing either NS or KLF7 shRNAs. (Scale bar: 500 μm.) (B) Average colony size of the data shown in A. (C) PDAC cell lines expressing NS or KLF7 shRNAs were injected s.c. into athymic nude mice (n = 5) and analyzed for tumor formation. The average tumor volumes at the indicated time points are shown. (D) Firefly luciferase-labeled PANC1 cells expressing NS or KLF7 shRNAs were injected into mice via the tail vein (n = 5). Representative bioluminescence images taken 1 wk and 4 wk after injection are shown. (E) Quantitation of bioluminescence in the mice at the indicated time points. (F) Representative images of hematoxylin and eosin-stained lung sections from the week 4 groups shown in D. (G) Relative size of metastatic nodules in week 4 lungs with Firefly luciferase-labeled PANC1 cells expressing KLF7 shRNA compared with the NS shRNA-expressing metastatic nodules that were considered 100%. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.