Figure 5.

VEGF, HIF1a and EPO levels are increased after Vhl^−/− in photoreceptors. Tamoxifen was administered to the Pde6g^creERT2/+;Vhl^flox/flox mouse model at P7 for 3 days. (A) Immunoreactivity of VHL and DAPI in both the Pde6g^creERT2/+;Vhl ^flox/flox and Pde6g^creERT2/+;Vhl^−/− mouse retina. Immunofluorescence staining of VEGF (B), HIF1a (C) and EPO (D) in both the Pde6g^creERT2/+;Vhl^flox/flox and Pde6g^creERT2/+;Vhl^−/− mouse retina (white scale bar = 50 μm; blue = DAPI; green in A = anti-VHL; green in B = anti-VEGF; red = anti-HIF1a; green in D = anti-EPO).