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. 2019 Jan 28;28(10):1738–1751. doi: 10.1093/hmg/ddz012

Figure 1.

Figure 1

Characterization of skeletal muscle and bone of 5-day-old WT, mdx, dKO-Het and dKO-Hom mice. (A) H&E staining (200×) of different skeletal muscle tissue samples from each of the four groups of mice. Mild muscle necrosis and central nucleated muscle fibers are observed in the skull (lower jaw muscle), spine, femur and tibial skeletal muscle for mdx, dKO-Het and dKO-Hom mice. (B–D) MicroCT images of the proximal tibia (30 slices, top view), lumbar spine 6 (spine L6) and midshaft cortical bone of the femur from the four groups of mice. (E–I) MicroCT quantification of BV/TV, density, Tb.N, Tb.Th and Tb.Sp of the trabecular bone of the proximal tibia. No differences were found in BV/TV, BV density, Tb.N and Tb.SP between dKO-Hom, dKO-Het, mdx and WT mice. The Tb.Th of dKO-Hom mice was significantly higher than that of WT mice (*P < 0.05). (J–N) MicroCT quantification of BV/TV, density, Tb.N, Tb.Th and Tb.Sp of trabecular bone of spine L6 showed no difference between the dKO-Hom mice and other groups. (O). Femur Ct.Th was significantly higher in mdx and dKO-Het mice than in WT mice (*P < 0.05), whereas no significant differences were found between dKO-Hom mice and other groups. (P) dKO-Hom mice showed no differences in femur cortical bone density compared to WT, mdx and dKO-Het mice.